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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential

Fig. 4

Effect of culture conditions on hPSC-LSC morphology and p63α, ABCG2, and LGR5 expression. a Representative cell morphology and p63α/ABCG2 protein expression under different culture conditions, as demonstrated by IF. Scale bars, 100 μm. b ABCG2 mRNA expression under different conditions, analyzed with qRT-PCR in d21 cells. Data are presented as the mean + SD, n = 3 technical replicates from one sample, P > 0.05. c Characterization of LGR5 protein expression in relation to ABCG2 and ∆Np63 at d11, as well as after continued culture in CnT-07+ENRC or CnT-30 at d24. Cell nuclei counterstained with DAPI (blue). Scale bars, 50 μm. Data shown for the representative hESC line Regea08/017

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Stem Cell Research & Therapy

ISSN: 1757-6512