Fig. 2
From: Isolation and characterization of a new basal-like luminal progenitor in human breast tissue
NOTCH3-FZD7 signaling is required for luminal cell fate commitment of bipotent progenitors. a Experimental strategy to apply gain and loss of NOTCH3 Receptor (NR3) and FZD7 function in the bipotent (BP, EpCAM+CD49f+CD90+) and luminal-restricted (LRP, EpCAM+CD49f+CD90−) progenitors isolated from transduced green fluorescent protein-positive (GFP+) precultured breast reduction samples. Average number of mix and pure luminal colonies generated from the BPs lacking either NR3 (shNR3-BP, b, c) or lacking FZD7 expression (shFZD7-BP, d, e), and from LRPs either lacking NR3 (shNR3-LRP, f) or lacking FZD7 (shFZD7-LRP, g) expression, or shScrambled control (scr), is shown as bar graphs. h, i Bar graphs showing total number of pure luminal colonies generated from BPs overexpressing either N3ICD (N3ICD-BP) or full-length FZD7 (FZD7-BP) or empty vector (EV), total number of mix colonies generated from N3ICD-BP or FZD7-BP (j, k), or from LRPs overexpressing N3ICD (N3ICD-LRP) or FZD7 (FZD7-LRP) (l, m). (n = 3, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005)