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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Isolation and characterization of a new basal-like luminal progenitor in human breast tissue

Fig. 3

Wnt7A-induced differentiation of bipotent progenitors into luminal progenitors requires NOTCH3-FZD7 signaling. Bipotent progenitors (BPs) and the luminal-restricted progenitors (LRPs) were obtained from precultured breast reduction samples and placed in colony-forming assays treated with either vehicle control (Vehicle) or recombinant human Wnt7A (rhWnt7A). Average colony-forming cell (CFC) frequencyā€‰Ā±ā€‰SD in the BPs (a) and LRPs (b) are shown as bar graphs. cā€“h Transduced BPs and LRPs (shNR3, shFZD7, N3ICD, FZD7, scr, EV) as in Fig.Ā 2 were obtained and placed in CFC assays with or without rhWnt7A. The average luminal and mix CFC frequencyā€‰Ā±ā€‰SD from the transduced BPs is shown as bar graphs (cā€“f). The average CFC frequencyā€‰Ā±ā€‰SD of luminal colonies from the transduced LRPs are shown as bar graphs (g, h). (nā€‰=ā€‰3, *pā€‰ā‰¤ā€‰0.05, ****pā€‰ā‰¤ā€‰0.001)

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