Fig. 3From: Isolation and characterization of a new basal-like luminal progenitor in human breast tissueWnt7A-induced differentiation of bipotent progenitors into luminal progenitors requires NOTCH3-FZD7 signaling. Bipotent progenitors (BPs) and the luminal-restricted progenitors (LRPs) were obtained from precultured breast reduction samples and placed in colony-forming assays treated with either vehicle control (Vehicle) or recombinant human Wnt7A (rhWnt7A). Average colony-forming cell (CFC) frequencyāĀ±āSD in the BPs (a) and LRPs (b) are shown as bar graphs. cāh Transduced BPs and LRPs (shNR3, shFZD7, N3ICD, FZD7, scr, EV) as in Fig.Ā 2 were obtained and placed in CFC assays with or without rhWnt7A. The average luminal and mix CFC frequencyāĀ±āSD from the transduced BPs is shown as bar graphs (cāf). The average CFC frequencyāĀ±āSD of luminal colonies from the transduced LRPs are shown as bar graphs (g, h). (nā=ā3, *pāā¤ā0.05, ****pāā¤ā0.001)Back to article page