Fig. 4
From: Isolation and characterization of a new basal-like luminal progenitor in human breast tissue
NOTCH3+FZD7+ cells represent a basal-like luminal progenitor. a Experimental strategy for isolating bipotent progenitors (BPs) and their subsets based on NOTCH3 and FZD7 receptors expression is shown; NR3highFZD7+ (subset a), NR3−FZD7+ (subset b), NR3medFZD7+ (subset ab), and the double negative NR3−FZD7− (subset dn). b The average ± SD initial progenitor subtype frequency in the unseparated BPs are shown as bar graphs. Average ± SD frequency of colonies generated from subset “a” cells and representative immunofluorescent staining of the colonies for CD8/18 and CD14 (c, d), for subset “b” (e, f) and subset “ab” and “dn” (g, h) are shown. The lines represent 1000 μm, and DAPI staining is used to visualize the nucleus. i, j Input progenitor numbers present in the unseparated bipotent progenitors (b) is used to calculate the enrichment of each progenitor in the subsets “a” and “b”