Fig. 7

Wnt7A-responsive BLPs differentiate to generate LRPs. The basal-like luminal progenitors (BLPs or subset “a”), subset “ab” and subset “b” were isolates as in Fig. 4 and placed in colony assays treated with rhWnt7A. a–c The average ± SD frequency of luminal and mix colonies from each progenitor subtype is shown in bar graphs. d The NR3⁻CD90⁺ BPs and BLPs were obtained from 3 reduction samples, cultured in vitro, and percentage of NR3⁺CD90⁺ cells was determined by flow cytometry at each passage (P). In the case of BLPs, only 1 sample out of 3 successfully expanded. e BLPs and luminal-restricted progenitors (LRPs) were cultured in colony assays and frequency of CD90⁺ cells after 4 and 7 days (immature and mature colonies respectively) was determined by flow cytometry. f The immature (d4) and mature (d7) colonies were made into single-cells and placed in serial colony assays and the total colony numbers are depicted in the bar graphs. g Schematic showing the developmental process of bipotent progenitors (BPs) differentiation into luminal-restricted progenitors (LRPs). The bar graphs show the average values ±SD for 3 reduction samples. (*p ≤ 0.05; **p ≤ 0.01)