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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Modulation of osmotic stress-induced TRPV1 expression rescues human iPSC-derived retinal ganglion cells through PKA

Fig. 3

TRPV1 is stimulated by NaCl-induced hyperosmotic stress in hiPSC-derived RGCs. a Immunofluorescence staining of intracellular and surface TRPV1 in RGCs treated with the indicated concentrations of NaCl. DAPI used as nuclear stain. b ImageJ quantification of immunofluorescence intensity of intracellular and surface TRPV1 expression in RGCs treated with the indicated NaCl concentrations. Means from 3 independent measurements are shown with SD error bars. *P < 0.05, Student’s t test. c Western blot analysis of expression of TRPV1 and other ion channels in RGCs treated with the indicated concentrations of NaCl. GAPDH used as loading control. d qRT-PCR analysis of BDNF mRNA (top) and ELISA analysis of secretion of BDNF protein in RGCs treated with the indicated concentrations of NaCl for 6, 12, and 24 h. Means from 3 independent measurements are shown with SD error bars

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