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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Human amniotic mesenchymal stem cells and their paracrine factors promote wound healing by inhibiting heat stress-induced skin cell apoptosis and enhancing their proliferation through activating PI3K/AKT signaling pathway

Fig. 1

Identification of hAMSCs. a Representative photomicrograph of adherent hAMSCs with spindle shapes on plastic cell culture dish. b RT-PCR analysis for the expressions of markers in hAMSCs. hAMSCs specifically expressed various markers of Nanog, Oct4, CD105, CD29, and CD90, but not CD34 and CD133. Water was used as negative control. c Flow cytometry analysis of CD29, CD90, CD73, CD105, CD34, CD45, HLA-DR, and HLA-ABC expression in hAMSCs. The red lines represent the isotype control, and the blue lines represent the level of surface markers. d The hAMSCs were negative for HLA-ABC co-stimulatory molecules CD80, CD86, and CD40. e Immunofluorescence staining of the embryonic stem cell surface markers Oct4, SSEA-4, and Nanog in hAMSCs. f Multiple differentiation potential of hAMSCs. The hAMSCs were differentiated into matured adipocytes and osteocytes after incubation with adipogenic or osteogenic differentiation medium at the times indicated, respectively. Adipocytes and osteocytes differentiated from hAMSCs were determined by staining with Oil Red O and Alizarin Red, respectively

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