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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human amniotic mesenchymal stem cells and their paracrine factors promote wound healing by inhibiting heat stress-induced skin cell apoptosis and enhancing their proliferation through activating PI3K/AKT signaling pathway

Fig. 2

hAMSCs or hAMSC-CM accelerated wound closure by subcutaneously injected around the injured site in C57BL/6 mice. a Representative images of wounds after transplantations of PBS, hAMSCs, H-DMEM, and hAMSC-CM at day 0, day 7, day 14, and day 21. b Measurement of wound closure at different time points treated with PBS, hAMSCs, H-DMEM, or hAMSC-CM. The results showed that the wound closure was significantly increased in response to hAMSCs and hAMSC-CM (n = 5). The percentage of wound closure was calculated as (area of original wound − area of measured wound)/area of original wound × 100. *PBS group compared with hAMSCs group; #H-DMEM group compared with the hAMSC-CM group. c Whole-body fluorescent imaging analysis of PKH26-labeling in hAMSCs in vivo at day 0, day 7, day 14, and day 21. d Immunofluorescence images of the sections from day-7, day-14, and day-21 skin tissues in PKH26-labeled hAMSCs. hAMSCs were co-stained with MAB1281 (antibody to human-specific nuclei) and CD90 and imaged by confocal microscope. e Representative photomicrographs of H&E stained sections from day-7 and day-14 wounds injected with PBS, hAMSCs, H-DMEM, or hAMSC-CM. The arrows indicate the layers of keratinocytes. Normal skin was used as a control

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Stem Cell Research & Therapy

ISSN: 1757-6512