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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human amniotic mesenchymal stem cells and their paracrine factors promote wound healing by inhibiting heat stress-induced skin cell apoptosis and enhancing their proliferation through activating PI3K/AKT signaling pathway

Fig. 4

hAMSCs and hAMSC-CM inhibited heat stress-induced apoptosis of HaCAT and DFL cells. a Examination of apoptosis cells in HaCAT and DFL cells with normal medium (NM), hAMSCs, and hAMSC-CM after 43 °C 50 min heat stress. The apoptosis of cells was assessed by FACS after 24 h of treatment. b Quantitative analysis of the percentage of apoptotic cells as shown in a (n = 3). c The expression analysis of Bcl-2 and Bax in HaCAT cells of different groups by immunoblotting assay. d Western blot assay for Bcl-2 and Bax expression in DFL cells of different groups. Significance was measured using a two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001

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