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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Human amniotic mesenchymal stem cells and their paracrine factors promote wound healing by inhibiting heat stress-induced skin cell apoptosis and enhancing their proliferation through activating PI3K/AKT signaling pathway

Fig. 5

hAMSCs and hAMSC-CM inhibited heat stress-induced growth inhibition of HaCAT and DFL cells and promoted their proliferation. a Representative images of the cell proliferation assay treated with normal medium (NM), hAMSCs, and hAMSC-CM for 48 h after 43 °C 50 min heat stress. b Cell viability for 48 h after treatment with normal medium, hAMSCs, and hAMSC-CM using a CCK-8 assay. c The expression levels of PCNA and Ki67 in HaCAT cells of different groups by immunofluorescence staining. d Immunofluorescence staining for determining the expression levels of PCNA and Ki67 in DFL cells of different groups. e Western blot assay for PCNA expression in HaCAT cells treated with normal medium, hAMSCs, and hAMSC-CM for 48 h after 43 °C 50 min heat stress. f Western blot assay for PCNA expression in DFL cells treated with normal medium, hAMSCs, and hAMSC-CM for 48 h after 43 °C 50 min heat stress. HaCAT and DFL cells not treated at 43 °C for 50 min and cultured in normal medium were used as controls. Significance was measured using a two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512