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Table 1 Primers and conditions used for RT-PCR to detect gene transcripts in hAMSCs

From: Human amniotic mesenchymal stem cells and their paracrine factors promote wound healing by inhibiting heat stress-induced skin cell apoptosis and enhancing their proliferation through activating PI3K/AKT signaling pathway

Genes Sequence TM (°C) Size (bp)
GAPDH F:5′-CCACCCATGGCAAATTCCATGGCA-3′ 59 598
R:5′-TCTAGACGGCAGGTCAGGTCCACC-3′   
Nanog F:5′-CAATGGTGTGACGCAGGGAT-3′ 52 149
R:5′-TGCACCAGGTCTGAGTGTTC-3′   
OCT4 F:5′-ATCCCTGAACCTAGTGGGGA-3′ 59 480
R:5′-CACTCGGACCACATCCTTCT-3′   
CD105 F:5′-TCCTCCCAAGGACACTTGTA-3′ 57 244
R:5′-CGCCTCATTGCTGATCATAC-3′   
CD29 F:5′-CCGCGCGGAAAAGATGAAT-3′ 55 406
R:5′-AAATGTCTGTGGCTCCCCTG-3′   
CD90 F:5′-GAGGGAGGAAGAGCAGACCT-3′ 57 896
R:5′-CCTGGATCGGGTTATGATGGG-3′   
CD34 F:5′-GCAAGCCACCAGAGCTATTC-3′ 55 390
R:5′-GGTCCCAGGTCCTGAGCTAT-3′   
CD133 F:5′-ATCCTTTCCATTACGGCGGC-3′ 57 311
R:5′-CTCAAGGCACCATCCCGTG-3′   
  1. F Forward primer, R Reverse primer