Fig. 2

Liver organoid functional analysis. a Representative image of liver organoid culture of all cell lines and tested conditions after 72 h of mesenchymal condensation in a 24-well plate and area analysis overtime (n = 3, biological replicates; data displayed as mean ± SEM). b HE staining for III groups of all cell lines (bar = 50 μm). c RT-qPCR gene expression analysis of hepatic markers at day 12, normalized with 2D cultured iPS-derived hepatocyte at day 18 of differentiation protocol. d Normalized albumin secretion at day 6 and day 12. d Urea secretion at days 6 and 12. e AFP secretion at day 12 (n = 3, biological replicates; data displayed as great mean and individual scatter plots; one-way ANOVA with Tukey’s post hoc, *p < 0.05, **p < 0.01, ***p < 0.01)