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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Adult and iPS-derived non-parenchymal cells regulate liver organoid development through differential modulation of Wnt and TGF-β

Fig. 3

Liver organoid cell signaling analysis. a Representative western blotting gels for all evaluated proteins (b) pSMAD2/SMAD2 analysis. c p-ERK1/2/ERK1/2 analysis. d β-Catenin analysis. e Jagged-1 analysis. f p-SMAD1.5.7/SMAD1.5.7 analysis. g Albumin gene expression after IPP organoid culture in presence of TGF-β and Wnt antagonist and agonist for 10 days. Data was normalized with untreated iPP organoid. h Graphical representation of western blotting results (n = 6, biological replicates; data displayed as great mean and individual scatter plots; one-way ANOVA with Tukey’s post hoc, *p < 0.05, **p < 0.01, and ***p < 0.001)

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