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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Human amniotic epithelial cells improve fertility in an intrauterine adhesion mouse model

Fig. 8

hAECs promoted autophagy in hEnSCs in vitro. A The cell viability of H2O2-treated hEnSCs significantly decreased. B hEnSCs were cocultured with hAECs in a Transwell system. C After 2.5 h of H2O2 treatment and another 24 h of culture, hEnSCs shrank severely, but hAEC coculture repaired the cell morphology of hEnSCs damaged by H2O2. D Western blot analysis showed that p62 expression increased significantly in H2O2-treated hEnSCs and decreased in hEnSCs cocultured with hAECs. The relative expression of LC3-II/LC3-I was decreased in H2O2-treated hEnSCs and increased in hEnSCs cocultured with hAECs. The expression level of ER was downregulated in H2O2-treated hEnSCs but upregulated in hEnSCs cocultured with hAECs. The expression of VEGF, PCNA, and PR did not change prominently. E The grayscale values of the western blots were evaluated. The ratios of LC3-II/LC3-I were standardized to those of the control group. The protein levels of p62, PCNA, VEGF, ER, and PR were normalized to that of β-tubulin (n = 3; *p < 0.05; **p < 0.01; ***p < 0.001; NS, p ≥ 0.05)

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