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Table 1 Top 5 enriched GO terms for pHPL-ASCs (upregulated) and FBS-ASCs (downregulated) at each passage (P0–P5). Related to Fig. 1

From: The effect of medium supplementation and serial passaging on the transcriptome of human adipose-derived stromal cells expanded in vitro

Gene expression Domain P0 P1 P2 P3 P4 P5
Upregulated BP Regulation of cell proliferation Extracellular matrix organization Cell cycle process Anatomical structure development Animal organ development Multicellular organism development
Cellular developmental process Extracellular structure organization Cell cycle Multicellular organism development Multicellular organism development Extracellular structure organization
System development Multicellular organism development Chromosome organization System development System development Extracellular matrix organization
Regulation of developmental process Anatomical structure development Mitotic cell cycle process Developmental process Tissue development Anatomical structure development
Multicellular organism development System development Mitotic cell cycle Animal organ development Anatomical structure development System development
CC Proteinaceous extracellular matrix Proteinaceous extracellular matrix Chromosome Proteinaceous extracellular matrix Proteinaceous extracellular matrix Proteinaceous extracellular matrix
Extracellular matrix Extracellular matrix Chromosomal part Extracellular matrix Extracellular matrix Extracellular matrix
Extracellular region Extracellular region Chromosomal region Extracellular region part Focal adhesion Extracellular region part
Extracellular region part Extracellular region part Nuclear lumen Striated muscle thin filament Cell-substrate adherens junction Collagen trimer
Extracellular space Extracellular space Non-membrane-bounded organelle Muscle thin filament tropomyosin Cell-substrate junction Extracellular region
MF Glycosaminoglycan binding mRNA binding involved in posttranscriptional gene silencing Protein binding mRNA binding involved in posttranscriptional gene silencing Oxidoreductase activity, oxidizing metal ions Transcription factor activity, RNA polymerase ii core promoter proximal region sequence-specific binding
Ion binding Collagen binding Catalytic activity, acting on DNA mRNA binding Metalloendopeptidase activity Metalloendopeptidase activity
Platelet-derived growth factor-activated receptor activity Extracellular matrix structural constituent Carbohydrate derivative binding Growth factor binding [heparan sulfate]-glucosamine 3-sulfotransferase 3 activity Metal ion binding
Heparin binding Platelet-derived growth factor receptor binding Adenyl ribonucleotide binding Transforming growth factor beta-activated receptor activity Ionotropic glutamate receptor binding Cation binding
Sulfur compound binding Ion binding Adenyl nucleotide binding Oxidoreductase activity, oxidizing metal ions, NAD or NADP as acceptor Metallopeptidase activity Xylosyltransferase activity
Downregulated BP Cell proliferation Biological adhesion Anatomical structure morphogenesis Anatomical structure morphogenesis Cell adhesion Regulation of multicellular organismal process
Anatomical structure morphogenesis Cell adhesion Multicellular organismal process Developmental process Biological adhesion Developmental process
Circulatory system development Multicellular organism development System development Vasculature development Anatomical structure morphogenesis Anatomical structure development
Extracellular structure organization Anatomical structure development Cell adhesion Cardiovascular system development Signaling Multicellular organism development
Extracellular matrix organization Anatomical structure morphogenesis Developmental process Anatomical structure development Regulation of multicellular organismal process Anatomical structure morphogenesis
CC Extracellular region part Extracellular region Extracellular region part Extracellular region part Extracellular region part Extracellular region part
Extracellular space Extracellular region part Extracellular region Extracellular region Extracellular region Extracellular region
Extracellular matrix Cell periphery Extracellular space Extracellular space Extracellular space Extracellular space
Extracellular matrix component Plasma membrane part Extracellular matrix Proteinaceous extracellular matrix Extracellular matrix Proteinaceous extracellular matrix
Integral component of plasma membrane Cell surface Proteinaceous extracellular matrix Extracellular matrix Proteinaceous extracellular matrix Extracellular matrix
MF Insulin-like growth factor binding Cell adhesion molecule binding Growth factor binding Glycosaminoglycan binding Glycosaminoglycan binding Glycosaminoglycan binding
Collagen binding Glycosaminoglycan binding Receptor binding Sulfur compound binding Heparin binding Sulfur compound binding
Protein-lysine 6-oxidase activity Growth factor binding Extracellular matrix structural constituent Heparin binding Sulfur compound binding Receptor binding
Transition metal ion binding Receptor binding Glycosaminoglycan binding Extracellular matrix structural constituent Extracellular matrix structural constituent Extracellular matrix structural constituent
Protein binding Integrin binding Insulin-like growth factor binding Growth factor binding Growth factor binding Heparin binding