Table 2 Top 5 enriched GO terms for significantly up- and downregulated DEGs for FBS-ASCs between subsequent passages. Related to Fig. 2a
Gene expression | Domain | P0–P1 | P1–P2 | P2–P3 | P3–P4 | P4–P5 |
|---|---|---|---|---|---|---|
Upregulated | BP | Cell migration | Immune system process | Protein-carbohydrate complex subunit organization | – | – |
Immune system process | Immune response | Polysaccharide assembly with MHC class II protein complex | – | – | ||
Leukocyte migration | Defense response | Protein-carbohydrate complex assembly | – | – | ||
Localization of cell | Response to stimulus | Antigen processing and presentation of polysaccharide antigen via MHC class II | – | – | ||
Cell motility | Inflammatory response | MHC class II protein complex assembly | – | – | ||
CC | Cell surface | Plasma membrane | MHC class II protein complex | – | – | |
Plasma membrane | Cell periphery | Lumenal side of endoplasmic reticulum membrane | – | – | ||
Cell periphery | Plasma membrane part | Integral component of lumenal side of endoplasmic reticulum membrane | – | – | ||
Integral component of membrane | Intrinsic component of plasma membrane | MHC protein complex | – | – | ||
Extracellular region | Integral component of plasma membrane | Crlf-clcf1 complex | – | – | ||
MF | Receptor binding | Receptor binding | MHC class II receptor activity | – | – | |
Chemokine activity | Receptor activity | MHC class II protein complex binding | – | – | ||
Receptor activity | Molecular transducer activity | MHC protein complex binding | – | – | ||
Cytokine activity | Peptide antigen binding | Peptide antigen binding | – | – | ||
Molecular transducer activity | Chemokine activity | Leptomycin b binding | – | – | ||
Downregulated | BP | System development | Multicellular organism development | Protein-carbohydrate complex subunit organization | Spliceosomal complex disassembly | – |
Multicellular organism development | System development | Polysaccharide assembly with MHC class II protein complex | Ribonucleoprotein complex disassembly | – | ||
Developmental process | Anatomical structure development | Protein-carbohydrate complex assembly | – | – | ||
Anatomical structure development | Developmental process | Antigen processing and presentation of polysaccharide antigen via MHC class II | – | – | ||
Tissue development | Anatomical structure morphogenesis | MHC class II protein complex assembly | – | – | ||
CC | Vesicle | Extracellular region part | MHC class II protein complex | U2-type post-mRNA release spliceosomal complex | – | |
Extracellular region | Extracellular region | Lumenal side of endoplasmic reticulum membrane | Post-mRNA release spliceosomal complex | – | ||
Extracellular region part | Extracellular space | Integral component of lumenal side of endoplasmic reticulum membrane | U2-type spliceosomal complex | – | ||
Extracellular space | Cell periphery | MHC protein complex | – | – | ||
Cell periphery | Plasma membrane | Crlf-clcf1 complex | – | – | ||
MF | Glycosaminoglycan binding | Cell adhesion molecule binding | MHC class II receptor activity | – | – | |
Cell adhesion molecule binding | Receptor binding | MHC class II protein complex binding | – | – | ||
Heparin binding | Cadherin binding | MHC protein complex binding | – | – | ||
Sulfur compound binding | Heparin binding | Peptide antigen binding | – | – | ||
Fibronectin binding | Growth factor binding | Leptomycin b binding | – | – |