The effect of medium supplementation and serial passaging on the transcriptome of human adipose-derived stromal cells expanded in vitro

Dessels, Carla; Ambele, Melvin A.; Pepper, Michael S.

doi:10.1186/s13287-019-1370-2

Stem Cell Research & Therapy

Table 4 Top 5 enriched GO terms for significantly up- and downregulated DEGs for FBS-ASCs between P0 and subsequent passages. Related to Fig. 3a

From: The effect of medium supplementation and serial passaging on the transcriptome of human adipose-derived stromal cells expanded in vitro

Gene expression	Domain	P0–P1	P0–P2	P0–P3	P0–P4	P0–P5
Upregulated	BP	Cell migration	Immune system process	Immune system process	Immune system process	Immune system process
		Immune system process	Immune response	Defense response	Immune response	Immune response
		Leukocyte migration	Defense response	Immune response	Cell surface receptor signaling pathway	Defense response
		Localization of cell	Regulation of immune system process	Inflammatory response	Response to stimulus	Response to stimulus
		Cell motility	Cell surface receptor signaling pathway	Response to stimulus	Defense response	Cell surface receptor signaling pathway
	CC	Cell surface	Plasma membrane	Plasma membrane	Plasma membrane part	Intrinsic component of plasma membrane
		Plasma membrane	Cell periphery	Cell periphery	Intrinsic component of plasma membrane	Plasma membrane
		Cell periphery	Plasma membrane part	Plasma membrane part	Integral component of plasma membrane	Plasma membrane part
		Integral component of membrane	Intrinsic component of plasma membrane	Intrinsic component of plasma membrane	Plasma membrane	Integral component of plasma membrane
		Extracellular region	Integral component of plasma membrane	Integral component of plasma membrane	Cell periphery	Cell periphery
	MF	Receptor binding	Receptor activity	Receptor activity	Receptor activity	Receptor binding
		Chemokine activity	Molecular transducer activity	Molecular transducer activity	Molecular transducer activity	Receptor activity
		Receptor activity	Receptor binding	Chemokine activity	Receptor binding	Molecular transducer activity
		Cytokine activity	Chemokine activity	Receptor binding	Peptide antigen binding	Peptide antigen binding
		Molecular transducer activity	Chemokine receptor binding	Signaling receptor activity	Chemokine activity	Chemokine activity
Downregulated	BP	System development	Anatomical structure development	System development	Anatomical structure development	System development
		Multicellular organism development	Multicellular organism development	Multicellular organism development	Developmental process	Developmental process
		Developmental process	Anatomical structure morphogenesis	Cell adhesion	Multicellular organism development	Multicellular organism development
		Anatomical structure development	Nervous system development	Biological adhesion	System development	Anatomical structure development
		Tissue development	System development	Developmental process	Anatomical structure morphogenesis	Anatomical structure morphogenesis
	CC	Vesicle	Extracellular region	Extracellular region part	Extracellular region part	Extracellular region part
		Extracellular region	Extracellular region part	Proteinaceous extracellular matrix	Extracellular region	Cell periphery
		Extracellular region part	Cell periphery	Extracellular region	Proteinaceous extracellular matrix	Proteinaceous extracellular matrix
		Extracellular space	Extracellular space	Extracellular matrix	Extracellular matrix	Extracellular region
		Cell periphery	Extracellular matrix	Extracellular space	Extracellular space	Plasma membrane
	MF	Glycosaminoglycan binding	Cell adhesion molecule binding	Glycosaminoglycan binding	Sulfur dioxygenase activity	Cell adhesion molecule binding
		Cell adhesion molecule binding	Neuropilin binding	Heparin binding	Glycosaminoglycan binding	Cadherin binding
		Heparin binding	Transporter activity	Receptor binding	Heparin binding	Receptor binding
		Sulfur compound binding	Cadherin binding	Sulfur compound binding	Cell adhesion molecule binding	Neuropilin binding
		Fibronectin binding	Protein tyrosine kinase activator activity	Ion binding	Cadherin binding	Actin binding

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ISSN: 1757-6512

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