Fig. 3

a Percentages of PDGFR-α+ OPCs (day 34), O4+ pre-oligodendrocytes (days 45–47), GALC+ immature oligodendrocytes (day 59), and MBP+ mature oligodendrocytes (day 59) (n = 3, three independent experiments). b The representative immunocytochemistry images of oligodendroglial protein expressions of PDGFR-α, O4, GALC, and MBP (three independent experiments were done). c–h The mRNA levels of MBP, PLP1, SOX10, OLIG2, MAG, and MOG at day 59 of oligodendrocyte differentiation timeline (n = 3). i Representative images of cells migrating out of spheroid at 2, 24, and 48 h after attachment to the culture plate (three independent experiments were done). j Quantification of cell migration distance (n = 3, ANOVA followed by t test). (Scale bar = 100 μm; n = 15, t test). Error bars indicate SEM. *P < 0.05; ***P < 0.001