Fig. 4

Chrysosplenetin increases Wnt/β-catenin pathway target gene expression in hBMSCs. RT-pPCR was performed to assess the expressions of target genes of Wnt/β-catenin pathway. hBMSCs cells were seeded with OIM and Chrysosplenetin (10 μM) in the presence or absence of Noggin (10 μg/ml) or DKK1 (0.5 μg/ml) for 3, 7, and 14 days. Gene expression was normalized to GAPDH; a CTNNB1; b Transcription Factor 7 (TCF7); c Lymphoid Enhancer Binding Factor 1 (LEF1); d MYC (C-MYC); e cyclin D (CCND1); f c-JUN (JUN) (*p < 0.05, **p < 0.01, ***p < 0.001 relative to Chrysosplenetin treating group)