Fig. 1From: Sequential stimulation with different concentrations of BMP4 promotes the differentiation of human embryonic stem cells into dental epithelium with potential for tooth formationHuman embryonic stem (hES) cell differentiation culture protocols. After hES cells were suspension-cultured for 3 days, embryoid bodies (EBs) formed (day 0). The EBs were transferred to standard culture plates and attached for 2 days (day 2). 1 pmol/L (pM) BMP4 stimulated EBs for 4 days to obtain oral ectoderm (OE) (day 6). Next, the OE differentiated into dental epithelium (DE) on stimulation with 30 pM BMP4 (Day 8)Back to article page