Skip to main content


Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Sequential stimulation with different concentrations of BMP4 promotes the differentiation of human embryonic stem cells into dental epithelium with potential for tooth formation

Fig. 1

Human embryonic stem (hES) cell differentiation culture protocols. After hES cells were suspension-cultured for 3 days, embryoid bodies (EBs) formed (day 0). The EBs were transferred to standard culture plates and attached for 2 days (day 2). 1 pmol/L (pM) BMP4 stimulated EBs for 4 days to obtain oral ectoderm (OE) (day 6). Next, the OE differentiated into dental epithelium (DE) on stimulation with 30 pM BMP4 (Day 8)

Back to article page