Fig. 3From: Sequential stimulation with different concentrations of BMP4 promotes the differentiation of human embryonic stem cells into dental epithelium with potential for tooth formationA low BMP4 concentration stimulated hES cells to differentiate into Pitx1+ cells. qRT-PCR expression levels of Oct4 (a) and Pitx1 (b) genes. EBs were cultured in EB medium for 6 days, and OE was cultured with 1 pM BMP4. ##P < 0.01 vs. hES cells; **P < 0.01 vs. EB. c Immunostaining of OE, DAPI (blue), and Pitx1 (green). Scale bar, 50 μmBack to article page