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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Sequential stimulation with different concentrations of BMP4 promotes the differentiation of human embryonic stem cells into dental epithelium with potential for tooth formation

Fig. 3

A low BMP4 concentration stimulated hES cells to differentiate into Pitx1+ cells. qRT-PCR expression levels of Oct4 (a) and Pitx1 (b) genes. EBs were cultured in EB medium for 6 days, and OE was cultured with 1 pM BMP4. ##P < 0.01 vs. hES cells; **P < 0.01 vs. EB. c Immunostaining of OE, DAPI (blue), and Pitx1 (green). Scale bar, 50 μm

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