Fig. 2

Expression of PBX1 in transduced HF-MSCs increased proliferation capacity. a The cell morphologies of PBX1-transduced HF-MSCs (bar, 100 μm). b Cell proliferation curve of HF-MSCs^EGFP and HF-MSCs^PBX1. c Effects of PBX1 in the cell cycle distribution in HF-MSCs. d Percentages of cells in the G₁, S, and G₂ phases of the cell cycle and PIs (e) of HF-MSCs^EGFP and HF-MSCs^PBX1. f, g Western blot analysis the levels of phospho-AKT, phospho-GSK3β, cyclin D1, p16, p21, and β-catenin proteins in HF-MSCs^EGFP and HF-MSCs^PBX1. GAPDH and HISTONE were used as endogenous controls for equal loading. The value for HF-MSCs^EGFP in the control group transduced by lentiviral vector was set as 1. *P < 0.05; **P < 0.01