Fig. 4
Characterization of PBX1-induced pluripotent stem cells from HF-MSCs. a Transcript levels of endogenous PBX1 in HF-MSCs, HF-iPSCs, and hESCs-X01 were determined by qPCR. The value in HF-MSCs as the control group was set as 1.0. b After transduction with SOMK, HF-MSCs were harvested on days 0, 7, 14, and 21, and the expression of endogenous PBX1 was assessed by qPCR. Data are shown as fold induction compared with that at day 0. c The cell morphologies of transduced HF-MSCs were changed by reprogramming at 0 and 34 days after SOMKP transduction (bar, 500 μm). d Expression of endogenous pluripotency genes in hESCs and HF-iPSCsSOMKP relative to that in parental somatic cell populations, as determined by qPCR. Data are shown as fold induction compared with that in hESCs-X01. e HF-iPSCsSOMKP expressed TRA-1-60, TRA-1-81, SSEA-3, SSEA-4, OCT4, and NANOG, as shown by immunostaining (bar, 200 μm). f H&E staining of teratomas obtained from HF-iPSCsSOMKP injected into NOD-SCID mice revealed gland-like structures (endoderm), smooth muscle (mesoderm), and squamous epithelium (ectoderm; bar, 100 μm). g Karyotype analysis. HF-MSCs at passage 6 (left) and HF-iPSCsSOMKP at passage 12 (right) showed a normal 46XY karyotype. *P < 0.05; **P < 0.01; ***P < 0.001