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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: PBX homeobox 1 enhances hair follicle mesenchymal stem cell proliferation and reprogramming through activation of the AKT/glycogen synthase kinase signaling pathway and suppression of apoptosis

Fig. 5

PBX1 enhanced HF-iPSC generation and activated the NANOG promoter. a, b Quantification of the number of alkaline phosphatase-positive colonies 32 days after lentivirus vector-mediated transduction with SOMKP and SOMK vectors into HF-MSCs. c Expression of endogenous pluripotency genes in HF-iPSCsSOMK and HF-iPSCsSOMKP was assessed by qPCR. The value in iPSCsSOMK as the control group was set as 1.0. d, e Quantification of the number of alkaline phosphatase-positive colonies 32 days after lentivirus vector-mediated transduction by SOMK with scrambled shRNA and SOMK with shRNA against PBX1. f Transduced HF-MSCs were harvested on days 7, 14, 21, and 28 to assess the expression of endogenous NANOG by qPCR. Data are shown as fold induction compared with the SOMK control on day 7. g Dual-luciferase reporter gene assays were used to assess the activation of the NANOG promoter in transduced HF-MSCs on days 7, 14, 21, and 28. The value in SOMK-transduced HF-MSCs as the control group was set as 1.0. *P < 0.05; **P < 0.01; ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512