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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Bone marrow concentrate-induced mesenchymal stem cell conditioned medium facilitates wound healing and prevents hypertrophic scar formation in a rabbit ear model

Fig. 1

The rabbit ear HS model. BMC-induced MSC CM accelerated wound healing and prevented HS formation in our rabbit ear model. a Experimental design. Four groups of rabbits were used. MSC CM, BMC CM, or BMC-MSC CM was administered to the left ears. In one set of rabbits, the left ears were left untreated as a sham group. The right ears of all groups received a DMEM injection as an internal control. b Images of gross examination. c Histological analysis of wounds and scar elevation index (SEI) measurement. Cells were stained with hematoxylin and eosin. Original magnification × 40. The SEI was measured using cross-sections of the central area of the wound. d Masson trichrome staining was used for the evaluation of collagen fiber organization. e Effects of MSC CM, BMC CM, and BMC-induced MSC CM on α-SMA, collagen type Ι, and collagen III mRNA expression, as measured by quantitative polymerase chain reaction using GADPH as a housekeeping gene and internal standard control. The data are shown as means ± standard errors of the means. *P < 0.05; **P < 0.01; ***P < 0.001. MSC CM, mesenchymal stem cell-conditioned medium; BMC CM, bone marrow concentrate; DMEM, Dulbecco’s modified Eagle’s medium; L, left; R, right

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