Fig. 2

Effects of MSC CM, BMC CM, and BMC-induced MSC CM on cultured primary HSs. a qRT-PCR analysis of α-SMA, collagen I, collagen III, fibronectin, MMP-1, and MMP-13 in HSs treated for 72 h with different stimuli. Expression is reported relative to that of GAPDH. Each data point was normalized to GAPDH expression and then the value of the control (DMEM), which was arbitrarily set as 1. b, c Western blot analysis and quantitative data for α-SMA, collagen I, collagen II, fibronectin, MMP-1, and MMP-13 after treatment with DMEM, MSC CM, BMC CM, and BMC-induced MSC CM for 72 h. GAPDH served as a control for equal protein loading. Histogram summarizing the results in b. Results were from three independent experiments using cells from four patients with HSs. The data are shown as means ± standard errors of the means. *P < 0.05; **P < 0.01; ***P < 0.001