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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Bone marrow concentrate-induced mesenchymal stem cell conditioned medium facilitates wound healing and prevents hypertrophic scar formation in a rabbit ear model

Fig. 4

BMC-induced MSC CM attenuated the activation of TGF-β1-stimulated myofibroblasts in human hypertrophic scar-derived fibroblasts. a Flow cytometry analysis and quantitative data for α-SMA-positive cells after TGF-β1 stimulation for 24 h. b Expression of the α-SMA gene after TGF-β1 stimulation for 24 h. c Immunofluorescence staining for α-SMA expression in HSs stimulated with TGF-β1 for 24 h and treated with DMEM, MSC CM, BMC CM, and BMC-induced MSC CM for 72 h. Scale bars = 100 μm. DAPI, 4,6-diamidino-2-phenylindole; MSC CM, mesenchymal stem cell-conditioned medium; BMC CM, bone marrow concentrate; DMEM, Dulbecco’s modified Eagle’s medium; SMA, smooth muscle actin. d Flow cytometry analysis and quantitative data for α-SMA-positive cells after TGF-β1 stimulation for 24 h, followed by treatment with DMEM, MSC CM, BMC CM, and BMC-induced MSC CM for 72 h (n = 4). Values are denoted as means ± standard errors of the means. *P < 0.05, **P < 0.01

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