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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Umbilical cord/placenta-derived mesenchymal stem cells inhibit fibrogenic activation in human intestinal myofibroblasts via inhibition of myocardin-related transcription factor A

Fig. 3

TGF-β1-induced fibrogenic activation of HIMFs is MRTF-A/SRF dependent. HIMFs were pretreated with or without CCG-100602 at 5, 10, 20, and 40 μmol/L concentrations for 30 min prior to the addition of TGF-β1 (5 ng/mL) for 24 (qPCR) or 48 (Western blots) hours. a qPCR analysis of the relative mRNA expression of COL1A1, FN1, ACTA2, MRTFA, and SRF. The data were normalized to the GAPDH expression and expressed as relative values compared with the control (n = 3). b Representative Western blots show the protein expression of Procol1A1, FN, and α-SMA with GAPDH as a loading control. c Representative Western blots show the protein expression of MRTF-A and SRF in the nuclear extracts with histone deacetylase 1 (HDAC1) as a loading control of the nuclear fraction. d, e Quantitation of Procol1A1, FN, α-SMA, MRTF-A, and SRF from the Western blot analyses (n = 3). Data are expressed as the means ± SEM. #P < 0.05 versus the untreated control; *P < 0.05, **P < 0.01, and ***P < 0.001 versus the TGF-β1 treatment only

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