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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Umbilical cord/placenta-derived mesenchymal stem cells inhibit fibrogenic activation in human intestinal myofibroblasts via inhibition of myocardin-related transcription factor A

Fig. 7

UC-MSCs inhibit TGF-β1-induced phosphorylation of Smad2. HIMFs were treated with TGF-β1 (5 ng/mL) and co-cultured with or without UC/PL-MSCs at 1 or 2 × 105 cells/insert for 48 h. a Representative Western blots show the protein expression of phosphorylated Smad2 (p-Smad2) with total Smad2 and GAPDH as a loading control. b Quantitation of p-Smad2 from Western blot analyses (n = 4). Data are expressed as the means ± SEM. ##P < 0.01 versus the untreated control; **P < 0.01 versus the TGF-β1 treatment only. NS, not significant

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