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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Restore natural fertility of Kitw/Kitwv mouse with nonobstructive azoospermia through gene editing on SSCs mediated by CRISPR-Cas9

Fig. 3

HDR-mediated correction of WV point mutation. a DNA sequencing of the W and WV point mutation loci in the Kitw/Kitwv SSC cell line. Both two mutation sites showed two peaks. b Schematic of the Cas9-sgRNA targeting and gene correction via HDR in WV point mutation loci. Blue line labeled the sgRNA-targeting sequences and the mutant base was marked in red. c DNA sequencing of the WV point mutation loci in repaired Kitw/Kitwv SSC cell line showed HDR-mediated repair gene-editing event. d DNA sequencing of the WV point mutation loci in typical error-repaired Kitw/Kitwv SSC cell line showed NHEJ-mediated “CACG” deletion. e Methylation analysis of the DMRs of H19 and Snrpn in Kitw/Kitwv SSCs and WV point mutation repaired SSCs. The result indicated that the repaired SSCs maintained paternal genomic imprints. Open and filled circles represent un-methylated and methylated CpG sites, respectively

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