Fig. 3

HDR-mediated correction of WV point mutation. a DNA sequencing of the W and WV point mutation loci in the Kit^w/Kit^wv SSC cell line. Both two mutation sites showed two peaks. b Schematic of the Cas9-sgRNA targeting and gene correction via HDR in WV point mutation loci. Blue line labeled the sgRNA-targeting sequences and the mutant base was marked in red. c DNA sequencing of the WV point mutation loci in repaired Kit^w/Kit^wv SSC cell line showed HDR-mediated repair gene-editing event. d DNA sequencing of the WV point mutation loci in typical error-repaired Kit^w/Kit^wv SSC cell line showed NHEJ-mediated “CACG” deletion. e Methylation analysis of the DMRs of H19 and Snrpn in Kit^w/Kit^wv SSCs and WV point mutation repaired SSCs. The result indicated that the repaired SSCs maintained paternal genomic imprints. Open and filled circles represent un-methylated and methylated CpG sites, respectively