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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: miR-106b regulates the proliferation and differentiation of neural stem/progenitor cells through Tp53inp1-Tp53-Cdkn1a axis

Fig. 1

The temporal expression patterns of miR-106b correspond with the decline of proliferating NPC pool. a A schematic representation of the sample collection during brain development. b Hierarchical cluster analysis of 44 miRNAs expressed during cortical development. c Sequence comparison of miRNAs encoded by the miR-17~106 family. d qPCR analysis of each miRNA in miR-17~106 family in E14 and adult mouse cortexes. e The expression levels of the miR-106b decreased with time during brain development, positively correlated with that of transcripts corresponding to proliferating NPC markers, Nestin and Ki67. f qPCR analysis of expression levels of miR-106b in Nestin-GFP+ and Nestin-GFP cells. g A schematic representation of the enrichment and differentiation of NPCs. h The enrichment and differentiation of NPCs was confirmed by immunoreactivities corresponding to NPCs (Nestin/Ki67) and differentiated cells (Tuj1/GFAP), respectively. i The expression levels of miR-106b decreased during NPCs’ differentiation, positively correlated with that of transcripts corresponding to Nestin and Ki67. Scale bar, 20 μm (g). Data are mean ± SD. p < 0.0001, p < 0.001, p < 0.01, and p < 0.05

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