Fig. 3

miR-106b inhibits the differentiation of NPCs. a A schematic representation of miR-106b LOF approach. b qPCR analysis of expression levels of miR-106b and transcripts corresponding to markers of NPCs (Nestin) and differentiated cells (Tuj1/GFAP) in the miR-106b LOF groups, compared to controls. c Immunofluorescence analysis of transduced cells displaying proliferating NPC (Ki67/Nestin)- and differentiated cell (Tuj1/GFAP)-specific immunoreactivities. d Quantification of cells displaying immunoreactivities corresponding to proliferating NPCs and differentiated cells in the miR-106b LOF group, compared to controls. e A schematic representation of miR-106b GOF approach. f qPCR analysis of expression levels of miR-106b and transcripts corresponding to markers of NPCs (Nestin) and differentiated cells (Tuj1/GFAP) in the miR-106b GOF group, compared to controls. g Immunofluorescence analysis of transduced cells displaying proliferating NPC (Ki67/Nestin)- and differentiated cell (Tuj1/GFAP)-specific immunoreactivities. h Quantification of cells displaying immunoreactivities corresponding to proliferating NPCs and differentiated cells in the miR-106b GOF group, compared to controls. Scale bar, 50 μm (c, g). Data are mean ± SD. ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, and ∗p < 0.05. Experiments were carried out three times in triplicates for in vitro perturbation