Fig. 4

miR-106b regulates the maintenance of NPCs in vivo. a A schematic representation of miR-106b LOF approach in vivo. b qPCR analysis of expression levels of miR-106b in the miR-106b LOF and control groups, compared to their respective controls. c qPCR analysis of transcripts corresponding to markers of NPCs (Nestin) and proliferating cells (Ki67) in the miR-106b LOF and control groups. (d, e) Immunofluorescence analysis and quantification of transduced cells displaying Sox2- (d) and EdU (e)-specific immunoreactivities. Scale bar, 10 μm (d, e). Data are mean ± SD. ∗∗∗p < 0.001, ∗∗p < 0.01, and ∗p < 0.05. Experiments were carried out three times in triplicates with 5–7 E14 embryos per group three times in triplicates for in vivo perturbation