Fig. 5

miR-106b targets Tp53inp1-Tp53-Cdkn1a axis in NPCs. a The GO analysis of top 20 enriched biological processes in the predicted miR-106b target genes. b, c qPCR analysis of candidate miR-106b target genes in the miR-106b LOF (b) and GOF (c) groups, compared to their respective controls. d The predicted consequential pairing of 3′UTR of candidate genes (top) and miR-106b (bottom) on the TargetScan website. e, f Repression of luciferase activities by the Tp53inp1 (e) and Cdkn1a (f) 3′UTR were dependent on miR-106b. Firefly luciferase activities were normalized to the internal control, Renilla luciferase activities. Data are mean ± SD. ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, and ∗p < 0.05. Experiments were carried out three times in triplicates for in vitro perturbation