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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: miR-106b regulates the proliferation and differentiation of neural stem/progenitor cells through Tp53inp1-Tp53-Cdkn1a axis

Fig. 6

ah miR-106b promotes the proliferation of NPCs through Tp53inp1-Tp53-Cdkn1a axis. a A schematic representation of the experimental approach. b qPCR analysis of expression levels of miR-106b and transcripts corresponding to Tp53inp1 and Cdkn1a in all experimental groups versus controls. c, d The number and size of neurospheres were quantified in all experimental groups versus controls. e qPCR analysis of levels of miR-106b and transcripts corresponding to Ki67, Nestin, Sox2, Tp53inp1, and Cdkn1a in all experimental groups versus controls. f Immunofluorescence analysis of transduced cells displaying proliferating cells (Ki67)- and NPCs (Nestin/Sox2)-specific immunoreactivities in all experimental groups versus controls. g Immunofluorescence analysis of transduced cells displaying EdU immunoreactivities in all experimental groups versus controls. h Quantification of cells expressing Ki67/Nestin/Sox2/EdU immunoreactivities in all experimental groups versus controls. Scale bar, 400 μm (b) and 20 μm (e, f). Data are mean ± SD. p < 0.0001, p < 0.001, p < 0.01, and p < 0.05. ####p < 0.0001, ###p < 0.001, ##p < 0.01, and #p < 0.05 versus the miR-106b LOF groups. Experiments were carried out three times in triplicates for in vitro perturbation

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