Fig. 4

miR-205-5p directly inhibited ANGPT2 expression via its 3′-UTR. a Overlap of three miRNA target bioinformatic prediction algorithms. b Expression of predicted targets in normal endometrial stromal cells. Data from The Human Protein Atlas. c The RNA levels of CDH11, ANGPT2, PLCB1, KPNA1, and HIF1AN in cells and xenografts associated with miR-205-5p-overexpressed EC109 cells were analysed by qRT-PCR. d RNA sequence alignment between miR-205-5p and the 3′-UTR of ANGPT2 (left), and the effect of miR-NC and miR-205-5p on the activity of the luciferase reporter containing either wild type (WT) or mutant type (MT) in EC109 were tested by dual-luciferase reporter assay (right). e The ANGPT2 protein levels in EC109 and EC520 cells transfected by lentivectors and in the xenografted lesions established by these ectopic endometrial stromal cells were detected by the western blot analysis. miR-205, miR-205-5p. Error bars represent the mean ± SD of three independent experiments. **P < 0.01