Fig. 4
ASC infusion results in thrombi formation in the lungs which is not affected by preincubation with an anti-tissue factor antibody. Mice were treated with 1 × 106 cryopreserved ASCs intravenously and lungs were harvested 3 h thereafter. Some mice were infected with K. pneumoniae via the airways 1 h prior to ASC infusion. ASCs were preincubated with a blocking anti-tissue factor antibody or isotype control prior to infusion. a Representative photographs of H&E-stained tissue sections of infected and uninfected lungs at 4 h, showing thrombi formation in ASCs-treated mice (arrows); original magnification × 40. b Quantification of thrombus formation (0 = no thrombi, 1 = 1 thrombus, 2 = 2–5 thrombi, 3 = 6–10 thrombi, 4 = > 10 thrombi per microscopic section). Data are expressed as bars for panel b (mean with SD). N = 4 mice per group. *p < 0.05 versus the control group