Fig. 4

Evaluation of survival, proliferation, and the expression level of immunoregulatory molecules of IL33-MSCs. MSCs were transfected with Vector-MSCs or IL33-MSCs for 48 h. Apoptosis was analyzed by Annexin V-PE/7-AAD staining and flow cytometry. A scatter diagram of apoptosis and histogram of apoptosis are shown in a. b, c IL-33 had no influence on MSC proliferation and viability as determined by EdU and CCK 8 respectively (n = 6). d Real-time PCR was used to determine the expression of IDO and COX2 in MSCs transfected with different plasmids (n = 4). e ELISA was used to determine the protein level of pGE2 in MSCs transfected with different plasmids (n = 4). *P < 0.05, ***P < 0.001, as indicated, ANOVA. Each experiment was performed three times