Table 1 Primer sequences used in quantitative and reverse transcriptase PCR assays
| Gene | primer sequence 5′-3′ |
|---|---|
| CD73 |
F: CTTAACGTGGGAGTGGAACC R: TCTAGCTGCCATTTGCACAC |
| CD90 |
F: CGCTCTCCTGCTAACAGTCTT R: CAGGCTGAACTCGTACTGGA |
| CD105 |
F: CTAACTGGCAGGGGAGACAG R: CTCCATGTGGCAGGAGCTA |
| CD166 |
F: ATTGAAGTTTTATTTGGCAGGAA R: GGCTTAGCCATGCAAAACA |
| CD34 |
F: TCTGGATCAAAGTAGGCAGGA R: GATCCAGCCTCAGAGGAAGA |
| CD45 |
F: GCAAAGATGCCCAGTGTTCCACTT R: ATCTGAGGTGTTCGCTGTGATGGT |
| CCND1 |
F: TATTGCGCTGCTACCGTTGA R: CCAATAGCAGCAAACAATGTGAAA |
| OCT4 |
F: CAGTGCCCGAAACCCACAC R: GGAGACCCAGCAGCCTCAAA |
| SOX2 |
F: CTCCGGGACATGATCAGC R: GGTAGTGCTGGGACATGTGAA |
| NANOG |
F: GCAGAAGGCCTCAGCACCTA R: GGTTCCCAGTCGGGTTCAC |
| PPARγ |
F: TCAGCGGGAAGGACTTTATGTATG R: TCAGGTTTGGGCGGATGC |
| RUNX2 |
F: GTCTTACCCCTCCTACCTGA R: TGCCTGGCTCTTCTTACTGA |
| COL2A1 |
F: CGTCCAGATGACCTTCCTACG R: TGAGCAGGGCCTTCTTGAG |
| FGF2 |
F: ATGGCAGCCGGGAGCATCACCCACG R: TCAGCTCTTCGCAGACATTGGAAG |
| TNF-α |
F: ATGAGCACTGAAAGCATGATCC R: GAGGGCTGATTAGAGAGAGGTC |
| VEGF |
F: TGCTTCTGAGTTGCCCAGGA R: TGGTTTCAATGGTGTGAGGACATAG |
| IL-6 |
F: AACCTGAACCTTCCAAAGATGG R: TCTGGCTTGTTCCTCACTACT |
| β-2-m |
F: CTCGCGCTACTCTCTCTTTCTG R: GCTTACATGTCTCGATCCCACT |