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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Mechanical process prior to cryopreservation of lipoaspirates maintains extracellular matrix integrity and cell viability: evaluation of the retention and regenerative potential of cryopreserved fat-derived product after fat grafting

Fig. 1

Characterization of the SVF gel. a Schematic diagram of SVF gel preparation. Lipoaspirates were centrifuged (1200×g, 3 min) to prepare Coleman fat (dense but intact fat without tumescent fluid and blood), which was transferred into a secured SVF gel Luer-lock syringe and passed through the syringe for shearing. The content of the syringe was centrifuged, and the resulting pellet was termed “SVF gel”. b Gross appearance. After cryopreservation for 1 month and thawing for 1 min, the cryo-fat pellet collapsed at room temperature and became covered by the released oil. However, the cryo-gel pellet maintained its shape. c Low-magnification scanning electron microscope (SEM) images. The cryopreserved SVF gel was enriched in ECM content, whereas the cryo-fat was immersed in oil and no clear micro-structure was observed. Scale bar = 100 μm. d Western blotting. The extracellular content was measured and compared between fresh fat and the SVF gel. The SVF gel contained a greater amount of ECM protein than the fat aspirate. However, a gradual loss of ECM protein (or antigen function) was observed in Col IV and fibronectin, but not in Col I. e Western blotting. The extracellular content was measured and compared between 1-month cryo-fat and 1-month cryo-gel. The cryo-gel contained a greater amount of Col IV and fibronectin than cryo-fat. n = 3 independent experiments with the fat of 3 donors

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