Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Mechanical process prior to cryopreservation of lipoaspirates maintains extracellular matrix integrity and cell viability: evaluation of the retention and regenerative potential of cryopreserved fat-derived product after fat grafting

Fig. 4

Fat grafting into an immunodeficient mouse model. a Gross appearance of the representative grafts. Cryopreserved human fat or SVF gel was transplanted subcutaneously into mice, and grafts were harvested after 3 months. Scale bar = 1.0 cm. b Weight retention. The SVF gel showed superior weight retention over the fat groups. Extended cryopreservation decreased the cryo-gel graft retention rate from 41.1 ± 0.141% (1-month group) to 24.1 ± 8% (3-month group), *p = 0.019, **pcryo-fat 1 month vs. cryo-gel 1 month = 0.016. The retention rate of the 1-month cryo-fat graft decreased markedly, barely reached one half of the retention of the 1-month cryo-gel (16.9 ± 0.077%). n = 6 mice. c H&E staining. The cryo-fat (1 month) graft had more oil droplets than the cryo-gel (1 month) graft. Cryo-fat (3 months) showed severe fat necrosis and increased oil cyst formation

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512