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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mechanical process prior to cryopreservation of lipoaspirates maintains extracellular matrix integrity and cell viability: evaluation of the retention and regenerative potential of cryopreserved fat-derived product after fat grafting

Fig. 5

Histological evaluation of the grafts. a Immunofluorescent staining. Viable adipocytes were labeled with Perilipin, and vasculature was labeled with vWF. Fresh fat and SVF gel grafts showed Perilipin+ mature adipose tissue. The 1-month cryo-gel, but not the 1-month cryo-fat, contained healthy adipose tissue. Adipogenesis was reduced in tissues frozen for 3 months. b FACS analysis of inflammatory cells. CD11b and F4/80 were used as pan-macrophage markers, and CD11b+ F4/80− cells were considered macrophage-depleted leukocytes. The fresh fat and SVF gel grafts had the lowest percentages of macrophage infiltration (5.88% and 6.46%, respectively), whereas the 1-month cryo-fat graft showed increased inflammation, with 12.38% of macrophages among SVF cells. After 3 months, the cryo-fat (1 month) graft showed prolonged macrophage activation, indicating chronic adipose tissue inflammation. n = 3 replicate

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Stem Cell Research & Therapy

ISSN: 1757-6512