Fig. 2

iPSC-derived MSC therapy increased infiltration of Treg in allograft. a Immunofluorescent staining for subepithelial human TSG-6 expression at d10 post transplantation. b Immunofluorescent staining for CD4⁺FOXP3⁺ lymphocytes at d10 post transplantation. c–e Morphometric analysis of graft infiltrating CD4⁺ T cells, FOXP3⁺ cells, and CD4⁺FOXP3⁺ co-localization (Tregs). Arrows highlighted individual CD4⁺ T cells (white arrows), FOXP3⁺ cells (blue arrows), and CD4⁺FOXP3⁺ Treg cells (yellow arrows). Data are presented as means with SE of 12 transplants/time point/experiments. *p < 0.05. Original magnification, × 40