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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: S100B is required for maintaining an intermediate state with double-positive Sca-1+ progenitor and vascular smooth muscle cells during neointimal formation

Fig. 1

Sca-1-positive progenitor cell migration and S100B expression during balloon injury-induced neointimal formation. The experiment preliminarily demonstrated the traits of distribution of both Sca-1+ progenitor cells and S100B expression in the vessel wall during the process of neointimal formation after vascular injury and identified the correlation between them. a Rats underwent carotid artery balloon injury. Representative sections of sham-operation and injured arteries at the indicated time points were stained with S100B. Open arrow indicates the neointima area, forked tail arrow indicates the media, and round arrow indicates the adventitia. b, c Semi-quantitative analysis of optical density value of immunohistochemical staining of S100B was determined within the media or intima of sham-operated and injured arteries by image-Pro Plus software. The percentage of S100B expression at the media or intima at the indicated time was calculated and compared to the sham group. d Representative whole image of immunohistochemical staining of S100B on the first and 14th day after injury. e Percentage of S100B levels at the vessel wall adventitia were determined at the indicated time and compared to the sham group by image-Pro Plus software. f Typical image of immunofluorescence staining with Sca-1 in sham-operated and injured arteries at the indicated time. Red fluorescence indicates Sca-1; blue fluorescence indicates DAPI-labeled nucleus. Round arrow indicates the neointima area, open arrow indicates the media, and forked tail arrow indicates the adventitia. g, h The number of Sca-1+ progenitor cells at the media or intima at the indicated times was calculated and compared to the sham group. i Representative whole image of immunofluorescence staining of Sca-1 cells on the first and 14th day after injury. Red fluorescence indicates Sca-1; blue fluorescence indicates DAPI-labeled nucleus. j The number of Sca-1+ progenitor cells at the vessel wall adventitia were determined at the indicated time and compared to the sham group by image-Pro Plus software. n = 6, #P < 0.05 compared to sham group; *P < 0.05 vs. first day after injury; &P < 0.05 vs. second day after injury; @P < 0.05 vs. seventh day after injury; $P < 0.05 vs. 14th day after injury

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