Fig. 2

S100B promoted Sca-1+ progenitor cell migration into the neointima through RAGE. Further experiments were designed to corroborate whether the increased S100B in injured vessels induced Sca-1+ progenitor cell migration into the neointima, by shRNA-induced SB100 knockdown in the injured vessels in vivo, using S100B neutralizing antibody or RAGE inhibitor to confirm the effect of S100B on Sca-1+ progenitor cell migration. a Representative image of fluorescence staining for Sca-1 in injured arteries treated with or without Ad-shS100B for 7 days. Round arrow indicates the neointima area, open arrow indicates the media, and forked tail arrow indicates the adventitia. Round arrow indicates the neointima area, open arrow indicates the media, and forked tail arrow indicates the adventitia. b, c The number of Sca-1+ progenitor cells were determined within the media or intima of a by image-Pro Plus software. n = 6, ^&P < 0.05 vs. the injured arteries treated with Ad-Null. d Typical image of response of human umbilical cord Sca-1 progenitor cell migration to conditioned medium (CM) of VSMC treated with or without Ad-S100B, following the addition of neutralizing antibody for S100B or RAGE inhibitor SPF-ZM1. e Quantitative analysis of cell migration was analyzed using the Transwell system. n = 15, *P < 0.05 vs. CM-Ctrl; ^#P < 0.05 vs. CM-Ad-S100B; ^&P < 0.05 vs. CM-Ad-S100B