Fig. 4
S100B and SDF-1α expression during balloon injury-induced neointimal formation. On the basis of the results of Fig. 1, this experiment preliminarily demonstrated the order of expressions of S100B and SDF-1α in the process of neointimal formation after vessel injury and tried to determine the correlation between expressions of S100B and SDF-1α. a Representational image of immunofluorescence double staining for SDF-1α and α-SMA in injured arteries at the indicated time points. Red fluorescence indicates SDF-1α, green fluorescence indicates α-SMA, and blue fluorescence indicates DAPI-labeled nucleus. Round arrow indicates lumen-side neointima, open arrow indicates the media, and forked tail arrow indicates the adventitia. b, c Semi-quantitative analysis of optical density value of immunohistochemical staining of SDF-1α within the media or intima of sham-operated and injured arteries was carried out using the image-Pro Plus software. The percentages of SDF-1α expression in the media or intima at the indicated time points compared to the sham group were calculated that showed time-dependent characteristics. d The dynamics of SDF-1α expression in VSMCs transfected with different MOI Ad-S100B as determined by western blot. e Semi-quantitative analysis of SDF-1α in the VSMCs from 4D. n = 3, &P < 0.05 vs. 30 MOI Ad-S100B; *P < 0.05 vs. 60 MOI Ad-S100B; $P < 0.05 vs. 80 MOI Ad-S100B