Fig. 5
S100B reduced Sca-1+ progenitor cell migration into the neointima through SDF-1α/CXCR4. The experiments were designed to determine whether increased SDF-1α in injured vessels induced Sca-1+ progenitor cell migration into the neointima by using SDF-1α receptor CXCR4 blocker AMD3100 to affirm the role of SDF-1α on Sca-1+ progenitor cell migration 7 days after vessel injury with or without AMD3100 application in vivo and 12 h after treatment in vitro. a Representational image of immunofluorescence double staining for Sca-1 and CXCR4 in injured arteries for 7 days. Red fluorescence indicates Sca-1, green fluorescence indicates CXCR4, and blue fluorescence indicates DAPI-labeled nucleus. b–d Typical image and semi-quantitative analysis of double positive with SDF-1α and α-SMA were determined within the media or intima of injured arteries treated with Ad-shS100B for 14 days, using the image-Pro Plus software. Red fluorescence indicates SDF-1α, green fluorescence indicates α-SMA, and blue fluorescence indicates DAPI-labeled nucleus. n = 6, &P < 0.05 vs. the injured arteries treated with Ad-Null. e Representative image of immunofluorescence staining of Sca-1 in the neointima of injured arteries treated with AMD3100. Red fluorescence indicates Sca-1, and blue fluorescence indicates DAPI-labeled nucleus. Round arrow indicates the neointima area, and open arrow indicates the media. f AMD3100 decreased the number of Sca-1+ progenitor cells in the neointima (panel e). n = 6, &P < 0.05 vs. Ad-Null. g Typical image of CXCR4 expression in hUCMSCs. Red fluorescence indicates CXCR4, and blue fluorescence indicates DAPI-labeled nucleus. h Cell migration mediated by conditioned medium (CM) with Ad-S100B was analyzed using the Transwell system following additions with or without of AMD3100. n = 15, *P < 0.05 vs. CM-Ctrl; &P < 0.05 vs. CM-Ad-S100B