Skip to main content
Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: S100B is required for maintaining an intermediate state with double-positive Sca-1+ progenitor and vascular smooth muscle cells during neointimal formation

Fig. 7

S100B induced SDF-1α expression in VSMCs through NF-kB signaling. The aim of the experiment was to explore possible molecular mechanisms of S100B-induced SDF-1α expression in VSMCs, using blockers for multiple signal pathways. a The indicated proteins and phosphorylated proteins as determined by western blot. b, c The SDF-1α expressions in VSMCs transfected with Ad-S100B, following the addition of ERK1/2 inhibitor PD98059 (PD), p38MAPK inhibitor SB203580 (SB), PI3K/Akt inhibitor wortmannin (WM), or NF-kB blocker (PDTC), as determined by western blot. n = 3, *P < 0.05 vs. Ad-Null; $P > 0.05 vs. Ad-Null; #P < 0.05 vs. Ad-S100B; &P < 0.001vs. Ad-S100B; @P < 0.001 vs. Ad-S100B. d Typical image of immunofluorescence staining for p52 in S100B-overexpressing VSMCs treated with or without PDTC or WM for 3 days. Green fluorescence indicates p52, and blue fluorescence indicates DAPI-labeled nucleus

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512