Fig. 7

Inflammation model of HK2 cells. High glucose levels (30 mmol/L glucose) and low levels of inflammatory cytokines (10 ng/mL rhTNF-α) were used to establish the inflammation model (GT) of HK2 cells in vitro. a CCK-8 assay of cell viability. b Flow cytometry of ROS production. c Flow cytometry of the cell apoptosis rate. d qRT-PCR analysis of mRNA expression levels. e Quantification of ROS production. f Quantification of the cell apoptosis rate. Each bar represents the mean ± s.e.m. Each group had three replicates per experiment, and all experiments in vitro were independently repeated three times unless indicated otherwise. *p < 0.05: GT versus the control; **p < 0.01: GT versus the control; ***p < 0.001: GT versus the control