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Table 1 Hemocytometer and blood smear analyses for the different cell isolation steps

From: Circulating adult stem and progenitor cell numbers—can results be trusted?

  Whole Blood (WB) DGC unwashed (DGCun) DGC washed (DGCw) RBCL washed (RBCL)
Blood cell counts (hemocytometer)
 WBC, 109/l 5.4 ± 0.8 0.7 ± 0.2***§§ 0.4 ± 0.1***‡‡§§ 3.1 ± 0.7***
 RBC, 1012/l 5.1 ± 0.4 0.0 ± 0.0*** 0.0 ± 0.0*** 0.0 ± 0.0***
 Hct, % 45.4 ± 3.0 0.0 ± 0.0*** 0.0 ± 0.0*** 0.0 ± 0.0***
 Hgb, g/dl 14.9 ± 1.2 0.0 ± 0.0*** 0.0 ± 0.0*** 0.0 ± 0.0***
 HDW, % 23.5 ± 2.0 35.7 ± 28.6 19.3 ± 26.4 28.2 ± 23.2
 RDW-CV, % 12.9 ± 0.5 17.9 ± 11.3 18.8 ± 19.4 23.3 ± 26.1
 MCV, fl 89.6 ± 3.2 70.5 ± 28.0 43.9 ± 30.6 39.0 ± 32.2
 MCH, pg 29.4 ± 1.2 0.0 ± 0.0*** 0.0 ± 0.0*** 0.0 ± 0.0***
 MCHC, g/l 328.2 ± 6.1 0.0 ± 0.0*** 0.0 ± 0.0*** 0.0 ± 0.0***
 Lymphocytes, 103/μl 1.75 ± 0.40 0.89 ± 0.23*** 0.70 ± 0.20** 0.76 ± 0.45*
 Monocytes, 103/μl 0.31 ± 0.06 0.18 ± 0.05§§§ 0.15 ± 0.05*‡§§§ 2.39 ± 0.39***
 Neutrophils, 103/μl 3.1 ± 0.4 0.0 ± 0.0*** 0.0 ± 0.0*** 1.1 ± 0.7**
 Basophils, 103/μl 0.02 ± 0.04 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
 Lymphocytes, % 32.2 ± 3.6 77.5 ± 2.5***§§§ 76.9 ± 5.3***§§§ 17.7 ± 11.8
 Monocytes, % 5.7 ± 1.1 16.1 ± 2.8**§§§ 17.0 ± 5.0*§§ 54.9 ± 6.4***
 Neutrophils, % 57.2 ± 4.3 1.5 ± 0.6***§ 3.0 ± 0.7***‡§ 24.0 ± 11.4**
 Basophils, % 0.6 ± 0.2 0.2 ± 0.1* 0.2 ± 0.2*** 0.2 ± 0.1*
Morphological parameters (blood smear)
 Lymphocytes, % 29.8 ± 4.7 77.9 ± 14.8 88.8 ± 3.7++ 27.4 ± 7.5
 Monocytes, % 7.0 ± 1.7 19.7 ± 15.5 7.3 ± 3.1+ 3.3 ± 3.2+++
 Rod-shaped granulocytes, % 2.8 ± 2.2 0.6 ± 0.6 0.0 ± 0.0 7.1 ± 6.2
 Segmented granulocytes, % 56.3 ± 6.8 0.3 ± 0.3 0.2 ± 0.4 46.0 ± 10.7
 Neutrophil granulocytes (rod-shaped + segmented), % 59.2 ± 7.8 0.83 ± 0.82 0.17 ± 0.41++ 53.1 ± 9.7+
 Basophil granulocytes, % 1.3 ± 1.0 1.3 ± 1.3 1.9 ± 1.4 0.8 ± 0.5
 Eosinophil granulocytes, % 2.7 ± 3.1 0.0 ± 0.0 0.0 ± 0.0 2.4 ± 1.7
 Debris, % 0.0 ± 0.0 0.0 ± 0.0 1.6 ± 3.9 13.0 ± 4.4
  1. Data are given as arithmetic mean ± standard deviation. n = 6; DGC density gradient centrifugation, RBCL red blood cell lysis, WBC white blood cell count, RBC red blood cell count, Hct hematocrit, Hb hemoglobin, RDW-CV red blood cell distribution width coefficient of variation, MCV mean corpuscular volume, MCH mean corpuscular hemoglobin, MCHC mean corpuscular hemoglobin concentration; significant differences to WB values and to RBCL are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001 and §p < 0.05, §§p < 0.01, §§§p < 0.001, respectively. Significant differences to DGCun are indicated as follows: p < 0.05, ‡‡p < 0.01. Significant differences between smear results and respective hemocytometer values are indicated as follows: +p < 0.05, ++p < 0.01, +++p < 0.001