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Table 1 Hemocytometer and blood smear analyses for the different cell isolation steps

From: Circulating adult stem and progenitor cell numbers—can results be trusted?

 

Whole Blood (WB)

DGC unwashed (DGCun)

DGC washed (DGCw)

RBCL washed (RBCL)

Blood cell counts (hemocytometer)

 WBC, 109/l

5.4 ± 0.8

0.7 ± 0.2***§§

0.4 ± 0.1***‡‡§§

3.1 ± 0.7***

 RBC, 1012/l

5.1 ± 0.4

0.0 ± 0.0***

0.0 ± 0.0***

0.0 ± 0.0***

 Hct, %

45.4 ± 3.0

0.0 ± 0.0***

0.0 ± 0.0***

0.0 ± 0.0***

 Hgb, g/dl

14.9 ± 1.2

0.0 ± 0.0***

0.0 ± 0.0***

0.0 ± 0.0***

 HDW, %

23.5 ± 2.0

35.7 ± 28.6

19.3 ± 26.4

28.2 ± 23.2

 RDW-CV, %

12.9 ± 0.5

17.9 ± 11.3

18.8 ± 19.4

23.3 ± 26.1

 MCV, fl

89.6 ± 3.2

70.5 ± 28.0

43.9 ± 30.6

39.0 ± 32.2

 MCH, pg

29.4 ± 1.2

0.0 ± 0.0***

0.0 ± 0.0***

0.0 ± 0.0***

 MCHC, g/l

328.2 ± 6.1

0.0 ± 0.0***

0.0 ± 0.0***

0.0 ± 0.0***

 Lymphocytes, 103/μl

1.75 ± 0.40

0.89 ± 0.23***

0.70 ± 0.20**‡

0.76 ± 0.45*

 Monocytes, 103/μl

0.31 ± 0.06

0.18 ± 0.05§§§

0.15 ± 0.05*‡§§§

2.39 ± 0.39***

 Neutrophils, 103/μl

3.1 ± 0.4

0.0 ± 0.0***

0.0 ± 0.0***

1.1 ± 0.7**

 Basophils, 103/μl

0.02 ± 0.04

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

 Lymphocytes, %

32.2 ± 3.6

77.5 ± 2.5***§§§

76.9 ± 5.3***§§§

17.7 ± 11.8

 Monocytes, %

5.7 ± 1.1

16.1 ± 2.8**§§§

17.0 ± 5.0*§§

54.9 ± 6.4***

 Neutrophils, %

57.2 ± 4.3

1.5 ± 0.6***§

3.0 ± 0.7***‡§

24.0 ± 11.4**

 Basophils, %

0.6 ± 0.2

0.2 ± 0.1*

0.2 ± 0.2***

0.2 ± 0.1*

Morphological parameters (blood smear)

 Lymphocytes, %

29.8 ± 4.7

77.9 ± 14.8

88.8 ± 3.7++

27.4 ± 7.5

 Monocytes, %

7.0 ± 1.7

19.7 ± 15.5

7.3 ± 3.1+

3.3 ± 3.2+++

 Rod-shaped granulocytes, %

2.8 ± 2.2

0.6 ± 0.6

0.0 ± 0.0

7.1 ± 6.2

 Segmented granulocytes, %

56.3 ± 6.8

0.3 ± 0.3

0.2 ± 0.4

46.0 ± 10.7

 Neutrophil granulocytes (rod-shaped + segmented), %

59.2 ± 7.8

0.83 ± 0.82

0.17 ± 0.41++

53.1 ± 9.7+

 Basophil granulocytes, %

1.3 ± 1.0

1.3 ± 1.3

1.9 ± 1.4

0.8 ± 0.5

 Eosinophil granulocytes, %

2.7 ± 3.1

0.0 ± 0.0

0.0 ± 0.0

2.4 ± 1.7

 Debris, %

0.0 ± 0.0

0.0 ± 0.0

1.6 ± 3.9

13.0 ± 4.4

  1. Data are given as arithmetic mean ± standard deviation. n = 6; DGC density gradient centrifugation, RBCL red blood cell lysis, WBC white blood cell count, RBC red blood cell count, Hct hematocrit, Hb hemoglobin, RDW-CV red blood cell distribution width coefficient of variation, MCV mean corpuscular volume, MCH mean corpuscular hemoglobin, MCHC mean corpuscular hemoglobin concentration; significant differences to WB values and to RBCL are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001 and §p < 0.05, §§p < 0.01, §§§p < 0.001, respectively. Significant differences to DGCun are indicated as follows: ‡p < 0.05, ‡‡p < 0.01. Significant differences between smear results and respective hemocytometer values are indicated as follows: +p < 0.05, ++p < 0.01, +++p < 0.001
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Stem Cell Research & Therapy

ISSN: 1757-6512